David, place the queen in her cage on top of the hive, cover off. Observe the bees. If they are biting at her, this isn't good, but this hive which we're talking about, if indeed is 100% queenless for fact, wants a queen to unite with. They will more than likely surround her and accept her, so I don't believe you will have a problem. I don't know the specifics of the hive in question, but I imagine you have done your homework, and have deduced that the hive is queenless and doesn't have a laying worker. Observe the bees as they surround the queen, if things look fine, then place her inside the hive, still in her cage. If you want to you can leave her in the cage for a couple of days or so, as long as she has attendants that are either in the cage with her or whereby the bees in the hive can attend to her. Not sure what type of queen cage she'll be in, do you know? Push in type, or plastic cage? After a few days you can remove the plug and let her out into the hive, things should be fine.
Sincerely, JP