If I dont lknow where the quen is, I give the frame a shake over the original hive. An easy shake.. JUST hard enough to dislodge SOME of the bees... If the queen is there she is usually the first to fall back into the original hive. At that point, it is also easier to look the frame over for the queen with less bees on it..
If you KNOW where the queen is, or you are confident she is not on the frame, there is no reason to remove the nurse bees. They will not fight when you put them into the hive. If your purpose is to strengthen a hive, taking a frame of eggs, larvae, and bees from a good strong hive is the way to go.
Even better, is if you keep a few nucs to take resources from.. you have spare queens, and you will have frames that NEED to be removed to keep the nucs from swarming, so you never have to steal anything from your production hives.