If you shoot them in the neck or the medulla oblongata (base of the brain, about the size of pencil eraser in a skunk) they won't twitch. I'm a good shot, but not that good. If they're traveling a consistant path you can put aspirin in eggs and the problem is solved. Note, the aspirin will take out other animals so know your target well. If something else (a pet) gets into it, Vitamin K is the antidote. Forcing them to drink hydrogen peroxide will cause them to throw up.
The skunks (mother and kit) I had this year have both disappeared, though I had nothing to do with it. I was 3 days into patterning them, for leaving the bait, when they left the scene on their own accord.
Wholly molly... I need to get a life.