I tried, and it worked. I had a refractometer for beer bought from . I used it. From my experience, I would advise you to use cold-pressed extra virgin olive oil. Set Brix scale to 71.5. Check calibration each day of use. I have found that the heated honey room (95-100 * F) seems to read higher moisture by around 2-3% falsely. Best to check at room temp I believe. The Brix measurement of a liquid varies according to its temperature. So, the measured Brix of a cold sample will count higher than a hot sample at room temperature. Ideally, you'll want your sample to be in the range of 10-30 ? C or 50-86 ? F.