This hive has given us problems before - most of ours bees don't mind us in their hive so much, but these girls fly up off the frames and headbutt us, and we're more likely to get stung with them than another hive.
(edit)
Ev'ning Phil...
Having had your colonies some years now you should be familiar with
managing a strong colony... like 80K occupants or more?
So when you are finding one which differs from your norm would it not bee
wise to look at why, first? Like eliminate any guesswork as to why they
are sensitive to disturbance?
OAD - observe assess determine.
It's too late now but some thought towards determination could have included
swapping queens. Were the aggressiveness to have moved you would then
know *know* the genetics are the problem, and so save yourself and the bees
a whole new dynamic in hard work.
For an enthusiaist there is no loss maybe in how you went about this excercise
but as an excercise in problem solving it is pointless, yeh?
Cheerio...
Bill