Hello Mr. Griggs. A lot of folks like to mark the queen, I do not. Very rare for me to mark a queen, the only reason is I don?t won?t to take a chance on hurting the queen. I agree a slim chance it is, but my experience is ANY damage to a queen is permanent. I realize a new beekeeper wants to easily spot the queen, but sounds like you very good at spotting this queen so why take a chance.
I do catch queens with my bare hands or nitrile gloves to give to beeks, so a little advice: always hold the queen by her thorax, her chest, never her head nor abdomen.
NEVER handle 2 queens with the same glove/hand. The first queen that touches the glove will leave her scent, the second queen detects this queen scent and will sting your glove or hand. I have not had a queen sting my hand why I pick one up and I cannot answer why.
One last note, Griggs you combined a laying worker hive with a queen right hive. Don?t do this, you take a chance the laying workers, false queens, or her associates will kill the queen in the queen right hive. Sounds like your combine worked though consider yourself lucky.
Blessings