The answer to your question is 'Yes'.
It sounds to me very much like the queen didn't have a clean break-away during mating and now has a blocked ovipositor. If it isn't that, it may be something similar.
The problem with this situation is that the queen will still smell 'right', and will be wandering around spreading her 'footprint pheromone' wherever she goes. From the colony's point-of-view everything appears 'hunky-dory', and so there's no reason to supercede her. Fortunately, by the installation of open brood combs, you've been preventing the development of laying workers, which would have resulted otherwise.
If it were me, I'd have her out of there, pronto - and try yet again with another frame of open brood.
My guess is you'll get lucky next time.
LJ