The population in my hives is noticeably increased, looks like doubled.
I agree with removing QE.
I think most well experienced Beekeepers do not use a QE.
I read for a worker to keep fighting her way through the QE can reduce their life by 50%.
Others, please correct me, if this is not correct.
Typically the Queen lays from the center of each frame outward in an arch and the workers will store honey at the top of frames.
If you just have one Brood Box, the Queen naturally does not cross the Honey line.
When you put on the second Brood Box, the workers will move the honey from top of frames in the lower Brood Box to the top of second box and then store the Honey in the Super when you add it.
In a natural hive, the Queen and the bees don't typically lay eggs where they store honey.