Well the experiment was a failure. I'm not exactly sure what happened. I checked on the "nucs" today and there were no bees in there at all. I noticed some robbing going on last evening, but I assume that's because the box was already abandoned. I did notice some dysentery spots earlier in the week, which may have been from a small beetle slimed section on a honey frame I gave to the one side that I didn't notice before. There was unhatched capped brood left which I think was dead, as it looked a little off. Maybe just not enough bees in the box?
As it's getting pretty late in the summer, I'm not going to try again. The queen whose mother I was questioning is doing extremely well, so I'm hopeful that she is a daughter of Guinevere, and I don't feel quite as concerned about losing the genes now. She is absolutely GIANT, the biggest queen I've ever seen! Thanks for all the help anyway, everyone. Hopefully Guin will make it through the winter, and I'll make some more splits from her in the spring.