The total mite fall in a 24 hour period is what you want to know. Usually the count is done each day for 3 days and then an average 24 hour count is obtained.
Counts that reach the point where the colony is having damage done varies from area to area. In my location a 24 hour average mite fall of 50 or above calls for the colony to be treated.
You may want to learn how to do an alcohol wash, this is the method now being used by most inspectors, that gives a number per 100 bees. Either will give an indication of when treatment is necessary.