Viking,
Next time you go to work on them, smoke them with just a few puffs of thick smoke in the Nuc and then wait a solid 10 minutes. The smoke them the same way and wait 30 seconds. Be sure not to band on the hive or on the platform, that can trigger a reaction from the bees.
There is a good chance that they have a lot of AFB in their genetics.
If they only chased you 50 feet their is a good chance they are not AHB. AHB will chase you a quarter mile.
Be very gentle handling the frames. It helps to keep them calm. Do not slam the frames back into the hive.
Jim