Ben, I gave the queens two brood cycles to be sure I was dealing only with their offspring, so I would say that 6 to 7 weeks after installing the queen will tell if there is an attitude problem. I had on hand nucs with queens I had raised in the spring, so it was just a matter of pinching the queen and installing the nuc. Those bees would meet me at the back door and often would sting before I walked the 50 yards to the apiary. I could work them with smoke and full protection, but my other colonies I was able to work with little or no smoke and no vail. Why have bees that are hard to work, a hobby is all about fun.